PCR-based Simple Sequence repeat (SSR) marker was employed to asses molecular diversity in twenty maize
inbreds lines. Eight out of the ten primers screened revealed polymorphism among the genotypes. Most of the
primers revealed single polymorphic band and average number of polymorphic band is 3.0 per locus.
Discrimination rate (DR) ranged from 0.23 (bnlg2328 and umc1304) to 0.83 (bnlg1520) with an average of 0.33.
Molecular analysis revealed a very high level of dinersity on the basis of PIC value among the lines, which
ranged between 0.43 (phi120) and 0.87 (mmc0063) with an average of 0.54. this clearly indicates high genetic
variability among the lines. Genetic similarity based on Jaccard’s similarity coefficient ranged from 0.22 to 0.72,
indicating narrow genetic variability among the genotypes based on SSR markers. The twenty maize inbreds
formed six clusters in the dendrogram in which I and II are major clusters and divided into subcluster. The
studies comprising twenty maize cultivars showed that association between dendrogram obtained by SSR
marker.