Mushroom cultivation represents, one of the economically viable processes for bioconversion of agricultural and industrial wastes into protein rich food making it a potent weapon against malnutrition in developing countries like India¶s. Strains were identified Pseudomonas, fluorescence, according to the characteristics of its morphology and homology analysis (BLAST) of its 16S-r DNA sequencing. In the present investigation collect 54 strains of bacteria from different rhizospheric soil. In selective media identified all strains of bacteria were found Actinomycetes, fluorescence and phasphate solublizing. In the Phasphate solublizing bacteria some strain of bacteria (P3, P4), were found which produces black colour in the nutrient broth medium 13-15 days after incubation period. This black colour was found due to secretion of some chemicals by bacteria. The experiment helped in isolating different strains fluorescence, actinomycetes and phosphate solublizing bacteria in rhizospheric soil from selected plants of different regions to examine the antagonistic agent against Pseudomonas agarici and to characterize the antagonistic effect in biochemical.