E. Turcicum is a fungal phytopathogen causing Turcicum Leaf Blight and responsible for a severe loss of yield in Maize. This work was aimed to characterize the bacterial population in the soil having antagonistic property against E. Turcicum, rhizospheric soil was analyzed and 16S rRNA method was employed for their molecular characterization. 100 soil samples were collected from rhizospheric soil of Rose, Kamini, Maize and Brassica from different regions of Banaras Hindu University. Subsequently 33 fluorescent bacteria were isolated on King’s B media. Isolates were detected on the basis of antagonistic property and 6 bacteria MBLK1, MBLK3, MBLK6, MBLK15, MBLK17, and MBLK22 were found to be antagonistic against TLB. Antagonistic bacteria were then analyzed for their plant growth promotion ability on Maize variety HUZ-M60. Afterwards, all of these antagonistic strains were tested on biochemical tests. The genomic DNA was isolated and amplified for 16s rRNA gene & these amplified segments were sequenced and compared for similar sequence with those available on database for public domain for identification of antagonistic fluorescent strains.