Present study was assessment genetic diversity of Colletotrichum species in chili pod, using Randomly Amplified
Microsatellites (RAM). Eight RAM primers were used to amplify genomic DNA of 17 isolated Colletotrichum species. These
samples were infected by C. capsici, C. acutatum and C. gloeosporioides, causing anthracnose disease. The DNA was
isolated and was amplified by thermo cycler after DNA quality tests, using RAM primer. These DNA fragments ranged from
250 bp – 5000 bp. On an average 3-9 bands appeared in per sample of DNA. Estimation of genetic distances (GD) ranged
from 16-63% was estimated