Vol.4 : Number 4
Genetic diversity of Colletotrichum species in chili using randomly amplified microsatellites (RAM)

Author(s): Saket Kumar, Vineeta Singh, Rajesh Singh, Major Singh


Present study was assessment genetic diversity of Colletotrichum species in chili pod, using Randomly Amplified Microsatellites (RAM). Eight RAM primers were used to amplify genomic DNA of 17 isolated Colletotrichum species. These samples were infected by C. capsici, C. acutatum and C. gloeosporioides, causing anthracnose disease. The DNA was isolated and was amplified by thermo cycler after DNA quality tests, using RAM primer. These DNA fragments ranged from 250 bp – 5000 bp. On an average 3-9 bands appeared in per sample of DNA. Estimation of genetic distances (GD) ranged from 16-63% was estimated


Country: India