Vol.4 : Number 5
Isolation of Trichoderma spp. and determination of their antagonistic, biochemical and plant growth promotion activity

Author(s): Vishal Tripathi, R. Singh, BK Sharma, HB Singh


Trichoderma spp. has been reported to be effective in controlling plant diseases, and the action of fungal hydrolytic

enzymes has been considered as one of the mechanism involved in the antagonistic process. In this study a total of 27

Trichoderma isolates were isolated from the rhizospheric soil samples of different plants collected from Mirzapur district

of Uttar Pradesh. The biocontrol activities of these isolates were studied by dual culture method against the phytopathogens

Rhizoctonia solani and Sclerotium rolfsii. Trichoderma isolate BM-4(ii) showed maximum inhibition (88.00%)

of Sclerotium rolfsii, whereas Trichoderma isolate BG-3(ii) showed the maximum inhibition (88.88%) of Rhizoctonia

solani. This effect might be attributed to the different mechanism of actions opted by different Trichoderma species.

Further all the 27 isolates were screened for the production of secondary metabolites. Out of the 27 isolates, all the

isolates produced cellulase, lipase, and amylase, 11 of them produced protease, 18 produced chitinase, and 17 produced

pectinase and phosphatase both. Selected isolates of Trichoderma were examined for plant growth promotion on Moong,

as a marked increase in the root length and shoot length was observed in comparison to control which was not treated with

Trichoderma. SDS PAGE analysis of the seedlings treated with Trichoderma showed that there was an increase in the

total protein content of the seedlings in comparison to the untreated control seedlings.

Key Words: Plant growth promotion, Secondary metabolite, SDS PAGE


Country: India