In an attempt to find out a standard and rapid protocol of micropropagation of Rose (Rosa indica L.) an experiment was
conducted with different type of explants with various combination of Murashige Stock culture media was used in the present
investigation. It was observed that rose explant with nodal segment was more responsive than shoot tip explants. One of the
problems with nodal explants was phenolics secretion, which retarded the growth of plant in cultured media and caused
browning of media. The problem was overcome by supplementing the some antioxidant and antiphenolic compounds like
Charcoal, PVP, AgNO3, etc. Increasing the concentration of cytokinine exerted positive effect on shoot proliferation and
addition of growth hormone also exerted positive effect on shoot proliferation. Some other factors such as pH, temperature
and light influenced the plant growth in media.
Key Words: Micropropagation, MS cultural media, Plant tissue culture, Rose