Vol.5 : Number 6
Rapid protocol for micropropagation of rose (Rosa indica L.)

Author(s): Pankaj Singh, SP Singh


In an attempt to find out a standard and rapid protocol of micropropagation of Rose (Rosa indica L.) an experiment was

conducted with different type of explants with various combination of Murashige Stock culture media was used in the present

investigation. It was observed that rose explant with nodal segment was more responsive than shoot tip explants. One of the

problems with nodal explants was phenolics secretion, which retarded the growth of plant in cultured media and caused

browning of media. The problem was overcome by supplementing the some antioxidant and antiphenolic compounds like

Charcoal, PVP, AgNO3, etc. Increasing the concentration of cytokinine exerted positive effect on shoot proliferation and

addition of growth hormone also exerted positive effect on shoot proliferation. Some other factors such as pH, temperature

and light influenced the plant growth in media.

Key Words: Micropropagation, MS cultural media, Plant tissue culture, Rose


Country: India