Biochemical and molecular characterization of fluorescent Pseudomonas from vindhyan region Santosh Kumar Yadav, Rajesh Singh, HB Singh

Biochemical and molecular characterization of fluorescent pseudomonas from vindhyan region

 

Santosh Kumar Yadav1, Rajesh Singh2, HB Singh3

1Forest Research Institute, Dehradun, India

2Genetics and Plant Breeding, IAS, BHU, Varanasi-221005

3Mycology and Plant Pathology, IAS, BHU, Varanasi-221005

ABSTRACT

 

Microbial diversity of vindhya region was explored especially of fluorescent Pseudomonas for their ability to produce different hydrolytic enzymes, inhibit phytopathogens and promote plant growth. Thirty one isolates of fluorescent Pseudomonas were isolated from rhizospheric soils of different plants from different locations of the Vindhya region on the basis of different parameters namely colony characteristic like colony shape, size, surface, margin and colour. Out of the 31 isolates, 30 isolates produced cellulase and amylase, 20 produced lipase, 28, 27, 21 isolates produced protease, phosphatase and pectinase, respectively in the medium. Out of the 31 isolates of fluorescent Pseudomonas 8 isolates showed antagonistic property against the pathogen Rhizoctonia solani and the percentage of inhibition ranged from 60.00-77.77%, whereas 6 isolates against the pathogen Sclerotium rolfsii and the percentage of inhibition ranged from 55.55-74.44%. Selected isolates of fluorescent Pseudomonas [BM-4(i), BM-5(iii), BC-9(i), BO-11(iv), BO15-(ii)] were examined for growth promotion activity on Moong (Phaseolus aureus) and a marked increase in shoot and root length was observed. Genomic DNA of 5 bacterial isolates were isolated from these found to be antagonistic against Rhizoctonia solani or Sclerotium rolfsii. The DNA samples (against a 100bp ladder sequences) were run in the agarose gel and the samples 2, 3, 4 and 5 showed clear bands in agarose gel. The samples showed clear visualizing bands containing adequate amount of DNA.

 

Key Words: Biochemical and Molecular Characterization, Pseudomonas, Vindhyan region