Screening of SCAR primer for pungency and non-pungency in chilli (Capsicum annuum L.)

Vivek Kumar Singh1, Kartikeya Srivastva2, Rajesh Singh2, Ved Prakesh Rai3

1The Energy and Resources Institute (TERI), India

2Genetics and Plant Breeding, IAS, BHU, Varanasi-221005

3Indian Institute of Vegetable Research, Varanasi-221005

ABSTRACT

The Chilli strains (Capsicum annuum L.) were screened for pungency and non-pungency using a set of nine primers in five different combinations. Two primers combinations of CSF-1 and CSR-2, CSF-1 and CSR-4 were found to be robust primers capable of very low capsaicin detection. The study indicated that the SCAR primers provide simple, fast and economic means of early detection of pungency in capsicum.

Key Words: Germplasm, Pungency, SCAR Markers