Genetic diversity of Colletotrichum species in chili using randomly amplified microsatellites (RAM)

Saket Kumar1, Vineeta Singh2, Rajesh Singh1, Major Singh3

1Genetics and Plant Breeding, IAS, BHU, Varanasi

2Dept. of Mycology and Plant Pathology, Institute of Agricultural Sciences (IAS), BHU, Varanasi

3Division of Crop Improvement and Biotechnology, Indian Institute of Vegetable Research (IIVR), Varanasi

 Received: 24 February 2015   Revised Accepted: 08 Apri 2015

ABSTRACT

Present study was assessment genetic diversity of Colletotrichum species in chili pod, using Randomly Amplified Microsatellites (RAM). Eight RAM primers were used to amplify genomic DNA of 17 isolated Colletotrichum species. These samples were infected by C. capsici, C. acutatum and C. gloeosporioides, causing anthracnose disease. The DNA was isolated and was amplified by thermo cycler after DNA quality tests, using RAM primer. These DNA fragments ranged from 250 bp – 5000 bp. On an average 3-9 bands appeared in per sample of DNA. Estimation of genetic distances (GD) ranged from 16-63% was estimated.

Key Words: Anthracnose, Chili, Colletotrichum species, Genetic Diversity, RAM