Rapid protocol for micropropagation of rose (Rosa indica L.) Pankaj Singh, SP Singh

Rapid protocol for micropropagation of rose (Rosa indica L.)

 

Pankaj Singh, SP Singh

Department of Horticulture, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005

 

Received: 13 January 2016                              Revised Accepted: 04 March 2016

 

ABSTRACT

 

In an attempt to find out a standard and rapid protocol of micropropagation of Rose (Rosa indica L.) an experiment was conducted with different type of explants with various combination of Murashige Stock culture media was used in the present investigation. It was observed that rose explant with nodal segment was more responsive than shoot tip explants. One of the problems with nodal explants was phenolics secretion, which retarded the growth of plant in cultured media and caused browning of media. The problem was overcome by supplementing the some antioxidant and antiphenolic compounds like Charcoal, PVP, AgNO3, etc. Increasing the concentration of cytokinine exerted positive effect on shoot proliferation and addition of growth hormone also exerted positive effect on shoot proliferation. Some other factors such as pH, temperature and light influenced the plant growth in media.

 

Key Words: Micropropagation, MS cultural media, Plant tissue culture, Rose