Rapid
protocol for micropropagation of rose (Rosa
indica L.)
Pankaj Singh, SP Singh
Department of Horticulture, Institute of
Agricultural Sciences, Banaras Hindu University, Varanasi-221005
Received: 13 January 2016 Revised
Accepted: 04 March 2016
ABSTRACT
In
an attempt to find out a standard and rapid protocol of micropropagation of
Rose (Rosa indica L.) an experiment
was conducted with different type of explants with various combination of
Murashige Stock culture media was used in the present investigation. It was
observed that rose explant with nodal segment was more responsive than shoot
tip explants. One of the problems with nodal explants was phenolics secretion,
which retarded the growth of plant in cultured media and caused browning of
media. The problem was overcome by supplementing the some antioxidant and
antiphenolic compounds like Charcoal, PVP, AgNO3, etc. Increasing
the concentration of cytokinine exerted positive effect on shoot proliferation
and addition of growth hormone also exerted positive effect on shoot
proliferation. Some other factors such as pH, temperature and light influenced
the plant growth in media.
Key Words: Micropropagation, MS cultural
media, Plant tissue culture, Rose