Expression of defense responsive gene in rice after the infection of R. solani

Raja Husain, NA Khan, KN Singh

Deptt. of Plant Molecular Biology & Genetic Engineering, Narendra Deva University of Agriculture & Technology Kumarganj Faizabad

Received: 27 September 2016                          Revised Accepted: 23 November 2016

ABSTRACT

Rice sheath blight, caused by Rhizoctonia solani is one of the major diseases of rice. The pathogen infects rice plants directly through stomata or using lobate appressoria and hyphal masses called infection cushions. Initially the mycelium looked thread like structure, milky white in colour than mycelium converted mature Sclerotia.  After the screening of different rice genotypes using 0 to 9 scaling (SES-IRRI), according to Leaf detach method, Pusa Basmati-1 was found highly susceptible variety and Swarna Sub-1 was susceptible one. Among wild rice, O. australiensis was observed resistant sps. While Oryza rufipogon was found Moderately Resistant sps. Approximately the same results were found in humidified chamber condition. During molecular analysis, in control condition, 8A1 gene/primer was not expressed with the cDNA transcript of Pusa Basmati-1 and Swarna sub-1, was expressed in stressed condition in both the cultivar with the product size 155 bp. In both wild rice namely O. rufipogon and O. australiensis, 8A1 gene was expressed in control as well as stressed condition with the product size 155 bp. While 4C₇ gene/primer was expressed continuously in control and stressed condition in susceptible rice cultivars; Pusa Basmati-1, Swarna sub-1 and wild rice accessions; O. rofipogon and O. australiensis with the product size 162 bp.

Key Words: Gene, Pathogen, Resistant, R. solani, Sheath blight