Standardiazation of regeneration protocol of ‘Canna indica’ in different nutrient media

Manoo Srivastava¹, Sudershan Kumar²

¹Sam Higginbottom University of Agriculture, Technology and Sciences Allahabad

²Principal scientist (Bioinformatics), National Botanical Research Institute (NBRI), Lucknow

Received: 12 October 2016                               Revised Accepted: 05 December 2016

ABSTRACT

Different nutrient media were used to test the totipotency of Canna indica. The Canna responded by exehibiting growth after the 7-12 days in presence of MS media. It was also observed that Canna grew fast through tissued cultured rasied bulbs and it was best, when at least 6-8 hours of sunlight each day is provided during the growing season. This protocol for plant tissue culture raised clone was standardized at National Botanical Research Institute (NBRI), Lucknow laboratories.

Key Words: Canna, Callus regeneration, Field condition, Growth regulators, MS media