Controling pod borer (heliocoverpa armigera) by natural toxin(s) in pigeon pea NA Khan, Raja Husain, Anamika Kaushal, Pratibha Singh, KN Singh

                                          Controling pod borer (Heliocoverpa armigera) by natural toxin(s) in pigeon pea


NA Khan1, Raja Husain1, Anamika Kaushal1, Pratibha Singh1, KN Singh2

1Department of Plant Molecular Biology & Genetic Engineering, Faizabad

2Department of Biochemistry, NDUA&T Kumarganj, Faizabad (U.P.) 224229


Received: 09 February 2017                             Revised Accepted: 13 April 2017




Among various constraints pigeon pea production, the insect pest has one of the prime importance. Helicoverpa armigera is one of the most devastating pests of this crop. Excessive use of chemical pesticides has resulted in environmental degradation, adverse effects on human health and other organism. For eradication of this insect, the researchers have shifted   from chemical to biological control. The naturally killed larvae of Helicoverpa were crushed and growth was tested  on LB agar plate, which  got the Helicoverpa larvae dead due to bacterial micro-organism and growth on PDA media indicated the presence of fungi. The protein profiling of bacterial protein on (SDS-PAGE) 12%, indicated very low molecular weight of protein which was responsible for death of Helicoverpa armigera. The ammonium sulphate fraction of bacterial protein was applied @ 30%, 60% and 70%   on live larvae. The rate of mortality was maximum in case of 70% ammonium sulphate fractionated protein.


Key Words: Ammonium Sulphate, PDA media, SDS-PAGE, Sephadex G-75