Controling pod borer (Heliocoverpa armigera) by natural toxin(s) in pigeon pea
NA Khan1, Raja Husain1,
Anamika Kaushal1, Pratibha Singh1, KN Singh2
1Department of
Plant Molecular Biology & Genetic Engineering, Faizabad
2Department of
Biochemistry, NDUA&T Kumarganj, Faizabad (U.P.) 224229
Received: 09 February 2017 Revised
Accepted: 13 April 2017
ABSTRACT
Among
various constraints pigeon pea production, the insect pest has one of the prime
importance. Helicoverpa armigera is
one of the most devastating pests of this crop. Excessive use of chemical
pesticides has resulted in environmental degradation, adverse effects on human
health and other organism. For eradication of this insect, the researchers have
shifted from chemical to biological
control. The naturally killed larvae of Helicoverpa
were crushed and growth was tested
on LB agar plate, which got the Helicoverpa larvae dead due to bacterial
micro-organism and growth on PDA media indicated the presence of fungi. The
protein profiling of bacterial protein on (SDS-PAGE) 12%, indicated very low
molecular weight of protein which was responsible for death of Helicoverpa armigera. The ammonium
sulphate fraction of bacterial protein was applied @ 30%, 60% and 70% on live larvae. The rate of mortality was
maximum in case of 70% ammonium sulphate fractionated protein.
Key Words: Ammonium Sulphate, PDA media, SDS-PAGE, Sephadex
G-75