Identification of phosphate solubilizing bacteria in different antagonistic bacterial strains Anand Kumar, RC Ram, Rajesh Singh

Identification of phosphate solubilizing bacteria in different antagonistic

bacterial strains

 

Anand Kumar1, RC Ram2, Rajesh Singh3

1School of Biochemical Engineering IIIT BHU Varanasi

2Mycology and Plant Pathology, IAS, BHU, Varanasi-221005

3Genetics and Plant Breeding, IAS, BHU, Varanasi-221005

 

Received: 18 March 2012                                                 Revised Accepted: 10 April 2012

 

ABSTRACT

 

Mushroom cultivation represents, one of the economically viable processes for bioconversion of agricultural and industrial wastes into protein rich food making it a potent weapon against malnutrition in developing countries. Strains  were identified  Pseudomonas,  fluorescence,  according  to  the  characteristics  of  its  morphology and  homology  analysis  (BLAST)  of  its  16S-r DNA sequencing.  In the present investigation collect 54 strains of bacteria from different rhizospheric soil. In selective media identified all strains of bacteria were found Actinomycetes, fluorescence and phasphate solublizing.  In  the  Phasphate  solublizing  bacteria  some  strain  of bacteria  (P3,  P4),  were  found  which  produces  black  colour  in  the  nutrient  broth  medium 13-15  days  after  incubation  period.  This  black  colour  was  found  due  to  secretion  of  some chemicals  by  bacteria. The experiment helped in isolating different strains fluorescence, actinomycetes and phosphate solublizing bacteria in rhizospheric soil from selected plants of different regions to examine the antagonistic agent against Pseudomonas agarici and to characterize the antagonistic effect in biochemical.

 

Key WordAntagonestic agent, Blast, Mushroom, Phosphate solublizing bacteria, 16s-r DNA