Identification of phosphate
solubilizing bacteria in different antagonistic bacterial strains
Anand Kumar1, RC Ram2,
Rajesh Singh3
1School
of Biochemical Engineering IIIT BHU Varanasi
2Mycology and Plant Pathology, IAS, BHU, Varanasi-221005
3Genetics
and Plant Breeding, IAS, BHU, Varanasi-221005
Received: 18 March 2012 Revised Accepted: 10 April 2012
ABSTRACT
Mushroom
cultivation represents, one of the economically viable processes for
bioconversion of agricultural and industrial wastes into protein rich food
making it a potent weapon against malnutrition in developing countries.
Strains were identified Pseudomonas, fluorescence, according
to the characteristics of
its morphology and homology
analysis (BLAST) of its 16S-r DNA sequencing. In the present investigation collect 54
strains of bacteria from different rhizospheric soil. In selective media
identified all strains of bacteria were found Actinomycetes, fluorescence
and phasphate solublizing. In the
Phasphate solublizing bacteria
some strain of bacteria
(P3, P4), were
found which produces
black colour in
the nutrient broth
medium 13-15 days after
incubation period. This
black colour was found
due to secretion
of some chemicals by
bacteria. The experiment helped in isolating different strains
fluorescence, actinomycetes and phosphate solublizing bacteria in rhizospheric
soil from selected plants of different regions to examine the antagonistic
agent against Pseudomonas agarici and to characterize the
antagonistic effect in biochemical.
Key Word: Antagonestic agent, Blast, Mushroom,
Phosphate solublizing bacteria, 16s-r DNA