A total twenty four inter-specific crosses of tomato developed by four susceptible and six resistant species in line x tester mating design. These crosses and their parents were evaluated in field and glasshouse of ICAR-Indian Institute of Vegetable Research, Varanasi, UP, India. The objective of study was to study the genetic nature of inter-specific crosses for resistance to Tomato leaf curl virus disease (ToLCVD) and yield traits under different environmental (field and glasshouse) conditions. In this study it was manifested that the disease pressure was more in glasshouse than field conditions due to high viral pressure by whiteflies. The performance of yield traits were good in field conditions. It was also found that the cross combination of EC-520061 were resistant for ToLCVD under both field and glass house environment. Whereas, the crosses of EC-521080, EC-528372, WIR-5032 and EC-528372 were better yield capacity. These crosses in resistant breeding programm. These crosses can be utilized and advanced for development of resistant varieties to ToLCV disease and high yield. 

Key Words:Eco-horticultural traits, Field, Glasshouse conditions, Heterosis breeding, Inter-specific crosses, Resistant to ToLCV, Wild spp.