ABSTRACT The bamboo plant is an important biodegradable raw material that can play an important role in the rejuvenation of the Indian rural economy by positively impacting agricultural, industrial, energy and environmental sectors. In vitro, bud break and aseptic culture establishment were evaluated for large-scale clonal propagation of the commercially important bamboo species Dendrocalams stocksii Munro. The experiment was conducted twice using a completely randomized design with 6 replicates per treatment. Among various concentrations of BAP and constant NAA, MS media supplemented with BAP 5 mg/L treatment is most congenial for the initiation and establishment of aseptic cultures. 6 Benzyl aminopurine (BAP) without supplementation of any additive resulted in significant bud breakage in the case of D. stocksii. Altered carbon source in MS media resulted in comparatively equal responses, and no significant variation was observed. For multiplication, BAP (5 mg/L) showed a maximum number of shoots (9±2) per explant in D. stocksii. For root initiation, further studies are needed. Well-developed plants were successfully acclimatized, and the success rate was 72%.

Keywords: Tissue culture, bamboo, BAP, NAA, Dendrocalamus stocksii Munro