R. solani is a disease of maize associated with low yields and the production of harmful mycotoxins therein. The disease is caused by the infection of host maize (Zea mays) with fungal pathogen R. solani. Moderately resistant maize cv. Kanchan was inoculated with conidial suspensions of R. solani or water (controls). Leaves of maize from each line were harvested after inculcation with R. solani. Further the biochemical and proteins were extracted from the inoculated as well as non-inoculated samples. After inoculation catalase and peroxidas activities 2-3 fold increased at different time of intervals. Maximum enzymes activities were recorded in between the 48 hpi to 96 hpi. The activity gel of catalase and peroxidise activity in control and after R. solani indicated that maize cv. Kanchan induces the defence response. The 2DE gel electrophoresis also showed some new proteins after inoculation with R. solani. Few proteins were also down-regulated in response to infection. Furthermore, peroxidise, β, 1-3-glucanase and induced proteins may be used as biochemical marker for rapid screening and resistance breeding in maize against R. solani.