Studies of molecular diversity in quality protein maize inbreds

Vinay Kumar1, PK Singh2, Rajesh Singh2, Astha Gupta3

1National Botanical Research Institute (NBRI) Lucknow, UP

2Genetics and Plant Breeding, IAS, BHU, Varanasi-221005

3National Botanical Research Institute (NBRI) Lucknow, UP

ABSTRACT

PCR-based Simple Sequence repeat (SSR) marker was employed to asses molecular diversity in twenty maize inbreds lines. Eight out of the ten primers screened revealed polymorphism among the genotypes. Most of the primers revealed single polymorphic band and average number of polymorphic band is 3.0 per locus. Discrimination rate (DR) ranged from 0.23 (bnlg2328 and umc1304) to 0.83 (bnlg1520) with an average of 0.33. Molecular analysis revealed a very high level of dinersity on the basis of PIC value among the lines, which ranged between 0.43 (phi120) and 0.87 (mmc0063) with an average of 0.54. this clearly indicates high genetic variability among the lines. Genetic similarity based on Jaccard’s similarity coefficient ranged from 0.22 to 0.72, indicating narrow genetic variability among the genotypes based on SSR markers. The twenty maize inbreds formed six clusters in the dendrogram in which I and II are major clusters and divided into subcluster. The studies comprising twenty maize cultivars showed that association between dendrogram obtained by SSR marker.

Key Words: Genetic relationship, Inbreds, Maize, PIC value, Polymorphism, Quality protein maize (QPM), SSR Marker