Molecular diversity of aspergillus in soil mycoflora of Eastern Uttar Pradesh

BK Pandey1, Asha Sinha2, Rajesh Singh3

1National Botanical Research Institute (NBRI) Lucknow, UP

2Mycology and Plant Pathology, IAS, BHU, Varanasi-221005

 3Genetics and Plant Breeding, IAS, BHU, Varanasi-221005

ABSTRACT

In the present experiment the universal primer ITS (Internal transcribed spacer) were used to access genetic relationships among three Aspergillus species viz, A. niger, A. flavus, A. candidus. Four ITS primer namely ITS-1, ITS-2, ITS-3 and ITS-4 were used to gain rapid and precise information about genetic similarities and dissimilarities of different Aspergillus species. Among all primers used ITS-1 and ITS-4 were more reproducible and show high level of polymorphism.

Key Words: Aspergillus niger, Alternaria alternat, Penicillium