Study of fluorescent bacteria antagonistic against Exserohilum turcicum

Kaushal Kumar Bhati1, Rajesh Singh2 

                              1National Agri-food Biotechnology Institute, Mohali

                                  2Genetics and Plant Breeding, IAS, BHU, Varanasi-221005

                                    Received: 28 March 2015   Revised Accepted: 26 May 2015

                                 ABSTRACT

E. Turcicum is a fungal phytopathogen causing Turcicum Leaf Blight and responsible for a severe loss of yield in Maize. This work was aimed to characterize the bacterial population in the soil having antagonistic property against E. Turcicum, rhizospheric soil was analyzed and 16S rRNA method was employed for their molecular characterization. 100 soil samples were collected from rhizospheric soil of Rose, Kamini, Maize and Brassica from different regions of Banaras Hindu University. Subsequently 33 fluorescent bacteria were isolated on King’s B media. Isolates were detected on the basis of antagonistic property and 6 bacteria MBLK1, MBLK3, MBLK6, MBLK15, MBLK17, and MBLK22 were found to be antagonistic against TLB. Antagonistic bacteria were then analyzed for their plant growth promotion ability on Maize variety HUZ-M60. Afterwards, all of these antagonistic strains were tested on biochemical tests. The genomic DNA was isolated and amplified for 16s rRNA gene & these amplified segments were sequenced and compared for similar sequence with those available on database for public domain for identification of antagonistic fluorescent strains.

Key Words: Antoun and Kloepper, Fluoresent pseudomonds