Molecular characterization of fluorescent bacteria antagonistic against turicum leaf blight Darshan Malik, Amit Srivastava

Molecular characterization of fluorescent bacteria antagonistic against

Turicum leaf blight


Darshan Malik1, Amit Srivastava2

1Department of Biochemistry, Shivaji College, University of Delhi, New Delhi, India

2Department of Genetics and Plant Breeding, Banaras Hindu University, Varanasi-221005


Received: 14 February 2017                             Revised Accepted: 18 April 2017




The objective of the present study was to characterize the bacterial population in the soil having antagonistic property against E. Turcicum, rhizospheric soil was analyzed and 16S rRNA method was employed for their molecular characterization. 100 soil samples were collected from rhizospheric soil of Rose, Kamini, Maize and Brassica from different regions. Subsequently 33 fluorescent bacteria were isolated on King’s B media. Isolates were detected on the basis of antagonistic property.  Antgonistic bacteria were then analyzed for their plant growth promotion ability on Maize variety HUZ-M 60. Afterwards, all the isolates were tested for gram staining, IMViC test, antibiotic assay and Indole acetic acid production test. IAA production was detected at different concentrations and it was noted that BMBK1 and BMBK6 showing the highest IAA production. Both the isolates were found to be Gram negative.


Key Words: Exserohilum turcicum, Entomophila L-48, Rhizospheric, Turcicum, 16S rRNA