Molecular characterization of fluorescent bacteria antagonistic
against
Turicum leaf blight
Darshan Malik1, Amit Srivastava2
1Department of Biochemistry,
Shivaji College, University of Delhi, New Delhi, India
2Department of Genetics and Plant
Breeding, Banaras Hindu University, Varanasi-221005
Received:
14 February 2017 Revised Accepted: 18 April 2017
ABSTRACT
The
objective of the present study was to characterize the bacterial population in
the soil having antagonistic property against E. Turcicum, rhizospheric
soil was analyzed and 16S rRNA method was employed for their molecular
characterization. 100 soil samples were collected from rhizospheric soil of
Rose, Kamini, Maize and Brassica from
different regions. Subsequently 33 fluorescent bacteria were isolated on King’s
B media. Isolates were detected on the basis of antagonistic property. Antgonistic bacteria were then analyzed for
their plant growth promotion ability on Maize variety HUZ-M 60. Afterwards, all
the isolates were tested for gram staining, IMViC test, antibiotic assay and
Indole acetic acid production test. IAA production was detected at different
concentrations and it was noted that BMBK1 and BMBK6 showing the highest IAA
production. Both the isolates were found to be Gram negative.
Key Words: Exserohilum turcicum, Entomophila
L-48, Rhizospheric, Turcicum, 16S rRNA